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KMID : 0613820100200010066
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Journal of Life Science
2010 Volume.20 No. 1 p.66 ~ p.70
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Genetic Relationship Based on RAPD Analysis of Yeosu Dolsan Leaf Mustard(Brassica juncea)
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Lee In-Ho
Park Jong-In
Jung Oon-Sup
Jung Hyo-Jin
Nou Il-Sup
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Abstract
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The genetic relationships of 14 Dolsan leaf mustard (Brassica juncea) lines were analysed using random amplified polymorphic DNA (RAPD) analysis. Thirty nine random primers were tested and seven primers which showed polymorphism were selected. The amplified fragments ranged from 0.2 to 3.5 kb in size. The number of bands amplified in each primer showed the variations ranging from 2 to 27, with an average of 8.7. Based on the UPGMA cluster analysis, 14 lines were separated into three groups. The results showed that RAPD analysis was useful in the genetic diversity and in the cross combination of the F1 hybrid of leaf mustard.
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KEYWORD
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Brassica juncea, RAPD, polymorphism
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